PARIS — Serena Williams lost in the second round of the French Open on Wednesday, her earliest exit at a major tournament since falling in the first round at Roland Garros two years ago.
Williams, the defending French Open champion and a 17-time major winner, lost to Garbine Muguruza of Spain 6-2, 6-2.
The loss came a short time after older sister Venus Williams also was eliminated in the second round. Venus lost to Anna Schmiedlova of Slovakia 2-6, 6-3, 6-4.
The sisters would have met in the third round had they both won.
This marked the eighth time in her last nine major tournaments that Venus failed to win more than one match.
The seven-time Grand Slam champion won the first set and was up an early break in the second against Anna Schmiedlova. But the 19-year-old Slovak responded by taking eight of nine games and won 2-6, 6-3, 6-4.
Since reaching the fourth round at Wimbledon in 2011, Venus has been eliminated in the first or second round at every Grand Slam tournament she has played except the 2013 Australian Open, where she reached the third round.
At 33 years old, Williams is far from her best. But the American has been having a solid season in 2014, winning a hard-court title in Dubai and reaching the final in Auckland.
Against Schmiedlova, the 29th-seeded Williams ended up with 47 unforced errors. Schmiedlova had only 20.
Also, 2012 French Open champion Maria Sharapova is scheduled to play Wednesday, as are Roger Federer and Novak Djokovic.
Venus won the last of her seven major titles at Wimbledon in 2008. She has never won the French Open, but lost to Serena in the 2002 final in their only meeting at Roland Garros.
On another cool day in Paris, both Venus and Schmiedlova were wearing an extra layer of clothing to keep warm.
Other winners included No. 12 Flavia Pennetta of Italy and No. 18 Eugenie Bouchard of Canada.
In the men's tournament, No. 18 Ernets Gulbis of Latvia, No. 27 Roberto Bautista Agut of Spain and No. 29 Gilles Simon of France advanced to the third round.
One match that carried over from the first round ended quickly Wednesday with Steve Johnson of the United States advancing. The American saved two match points on Tuesday in the third set before completing a 4-6, 6-7 (7), 7-6 (3), 6-3, 6-3 win over Laurent Lokoli of France.
The match had been suspended because of darkness on Tuesday night with Johnson leading 3-1 in the fifth.